Background: Postpartum deep venous thrombosis is a leading cause of maternal mortality and can carry significant long-term morbidity. Aim: was to investigate effect of implementing evidence-based nursing practices guidelines on prevention of deep venous thrombosis among postpartum women. Design: A quasi-experimental research design. Sample: A purposive sample of two groups (50 women in study group and 50 women in control group). Setting: This study was conducted at antenatal clinic and postpartum unit of Obstetrics and Gynecological Department in Benha University Hospital, Qaluobia, Egypt. Tools: Four main tools were utilized. Tool (I): A structured interviewing questionnaire. Tool (II): Deep vein thrombosis risk factors assessment sheet. Tool (III): Women's compliance with the DVT preventive measures. Tool (IV): Deep vein thrombosis assessment sheet. Results: There was highly statistically significant difference between study and control groups regarding their knowledge about DVT at post-intervention compared to pre- intervention. Additionally, there was a highly statistical significance difference among both groups post-intervention regarding to their compliance with DVT preventive measures. Moreover, a significant reduction in the occurrence and manifestations of DVT in the study groups compared to control groups with statistically significant difference post-intervention. Conclusion: Women who receive the Evidence-Based Nursing Practices Guidelines exhibit a higher level of knowledge regarding DVT and its preventive measures, as well as demonstrate good compliance with deep vein thrombosis preventive measures and had significant decrease in the occurrence of DVT and its associated symptoms in comparison to those who do not receive. Recommendation: Increased awareness regarding the risk factors and preventive measures for DVT should be incorporated as a vital component of routine antenatal care during the third trimester of pregnancy.