Background: One of the top five leading causes of women deaths worldwide is puerperal sepsis., preventive care bundle are the best nursing interventions based on evidence applied in clinical practice for prevention puerperal sepsis. Aim: The present research aimed to evaluate the effect of utilizing care bundle on prevention of puerperal sepsis among post-natal women. Design: A quasi-experimental research design (study/control group, pre/post-test) was utilized. Sample: A purposive sample of 100 women (pregnant women at 36 gestational weeks and followed immediately after delivery). Setting: The present research conducted at the out- patient clinic of obstetrics & gynecology department and postnatal room (recovery room) at Benha University Hospital. Tools: three tools utilized included: I- A structured interview questionnaire, II-part one -knowledge assessment questionnaire, part two-care bundle practices scale, III-the sepsis in obstetric score sheet. The results: there were highly statistical significance improvements found at two weeks after delivery and forty days postnatal in the total knowledge score of the study group compared to control ones (P ≤ 0.001). there was statistically significant difference between study and control group immediately after delivery in all elements of care bundles practices(Р-value<0.05), and there was high statistically significant difference in all elements of care bundles practices between study and control group at two week and forty day post-natal (P ≤ 0.001). there was no significant differences in the Sepsis in Obstetrics Score between the study and control groups at immediately after delivery (Р-value>0.05) while there was ahigh statistically significant difference at two weeks and forty days postnatal of the study group compared to control ones (P ≤ 0.001).Conclusion: the majority of the study group had good total knowledge scores and good total care bundle practices scores  for prevention of puerperal sepsis compared to less than the half of the control group at two weeks after delivery and 40 days postnatal with highly statistical significance  improvements of the study group compared to control group (P ≤ 0.001). Also, the most of study group had normal sepsis in obstetric scores compared to about two thirds of the control group with highly statistical significance improvements (P ≤ 0.001). Recommendations: Care bundle should recommend as evidence-based nursing interventions applied to postnatal women for prevention of puerperal sepsis.