Background: The interest of health organizations in organizational culture has increased due to many positive outcomes such as job satisfaction, and empowerment. Aim: Assess organizational culture and its influence on staff nurses' empowerment. Research design: A descriptive, correlational design was used. Setting: The study was conducted at Ain Shams University Hospital.  Subjects:  105 staff nurses out from 308 were included in the study. Tools: Data was collected by using an organizational culture inventory and, an empowerment questionnaire. Results: Less than one-third of the study participants had high perception level regarding organizational culture. While more than half of them had a moderate level of organizational culture. Moreover, more than two-fifths of them had a low level of empowerment. Conclusion: There was a highly statically significant positive correlation between staff nurses regarding of organizational culture and their empowerment. Recommendations: Encouraging nurses' participation in goal setting, problem solving, and decision making. Conduct a regular meeting between the supervisors and nurses to discuss and solve their problems.