Background: Coronavirus disease (COVID-19) is a deadly virus that continues to afflict many countries worldwide. The development of a COVID-19 vaccine to combat the disease's spread. Health Care Workers are major influencers in vaccination, thus their acceptance or hesitancy to tcovid-19 vaccine would either aid its acceptance among the populace. Aim of the study: Assess knowledge and attitude of healthcare workers regarding COVID 19 vaccination. Design: A descriptive study was utilized in this study. Setting: The current study was conducted in Ashmoun General Hospital, which is affiliated to the Ministry of Health and Population in Menoufia Governorate. Subjects of the study: The study subjects include 266 health care workers including physician, anaesthetist, nursing staff, pharmacist, dentist, physiotherapist, labs scientist, radiographer and housekeeping in the previous mentioned setting. Tools of data collection: Tool I. Self-administrated Questionnaire Sheet: It was developed by the investigator after reviewing the recent related literature included. Part I: It was concerned with demographic characteristics such as age, gender, marital status, educational level, job title, years of working experience etc...) Part II: It was used to assess level of knowledge of healthcare workers regarding COVID 19 vaccination. Tool II: It was used to assess level of attitude of healthcare workers regarding COVID 19 vaccination. Results of the study: Represents that two third of the studied sample had good total knowledge, nearly one quarter of them had average level but one tenth of them had poor total knowledge, and more than two third of studied sample had positive attitude regarding COVID-19 vaccination, one third of them had negative attitude. Conclusion: In the light of present study findings, the health care workers were very knowledgeable with positive attitude to COVID-19 vaccination. Recommendations: Continuous evaluation of health care workers knowledge and attitude regarding COVID 19 vaccination is essential to identify their needs.