Background: The recent spread of human Marburg viral infection outbreaks has posed a new public health challenge, underscoring the importance of conducting studies to enhance our understanding of infectious diseases and contribute to sustainable development goals. Aim: This study aimed to assess the effect of educational sessions on Marburg viral infection at Fever and Chest outpatient clinics in Mansoura Hospitals. Design: A quasi-experimental design with pre- and post-tests was employed for this research. Setting: The research was conducted at Fever and Chest outpatient and emergency clinics in Mansoura hospitals. Sampling: A convenience sample of 80 nurses, comprising 50 from Chest Hospital and 30 from Fever Hospital, was included. Data Collection Tools: Two instruments were used. Tool (I) consisted of a self-administered questionnaire divided into three sections: Nurses' characteristics, their knowledge, and practices related to Marburg viral infection. Tool (II) assessed nurses' attitudes toward Marburg viral infection. Results: Initially, 12.5% of the participating nurses demonstrated good knowledge about Marburg viral infection, which improved to 76.3% after the educational intervention. Furthermore, the overall level of practices increased from 47.5% pre-sessions to 87.5% post-implementation, with a slight decrease to 77.5% during follow-up. Similarly, the level of nurses' attitudes exhibited a positive change, with Positive Attitudes among 15% of nurses pre-intervention, increasing to 81.3% post-intervention (p<0.001). Conclusion: The implementation of educational sessions resulted in enhanced knowledge, practices, and attitudes of nurses regarding Marburg viral infection. Post-test scores for knowledge, practices, and attitudes were higher compared to pre-test scores. Recommendations: Continuous educational interventions are crucial to further enhance nurses' knowledge and practices, as effective outbreak control requires collaborative efforts from skilled healthcare professionals.