Background: Testicular cancer (TC) is the most common malignancy in young men between the ages of 15 and 35 years. It is easily treated if detected early through regular testicular self-examination (TSE). Raising students' awareness of testicular cancer and TSE is very important for early diagnosis and improving their health belief. Aim of the study: To evaluate the effect of educational booklet based on health belief model male nursing Students' knowledge, practice and beliefs regarding testicular self-examination. Methods: A quasi experimental design was used. A convenient sample of 112 male nursing students from Faculty of Nursing South Valley University. Tools: Three tools were used. I: A self-administered questionnaire that assessed demographics and testicular cancer knowledge. II: Testicular self-examination checklist to assess the nursing students' practice regarding TSE III: Champion's Health Belief Model Scale to assess the nursing students' beliefs and self-efficacy regarding TC and TSE. Results: There was a highly statistically significant improvement among male nursing students in their level of knowledge, practice and Health Belief Model about TSE at immediate post, and post 3 months after implementation of the educational booklet with p< 0.05. Also, there was a positive correlation between level of knowledge and practice with Health Belief Model in pre, post one month and post three months at p-value <0.01. Conclusion: The use of an educational booklet based on the health belief model has a beneficial effect on nursing students' knowledge and practice and health belief regarding TC and TSE. Recommendation: Regular TSE is essential for the detecting TC early. There for, it is crucial to conduct extensive teaching campaigns and training on TC and TSE techniques among younger male.