Background: There exists a gap between knowledge regarding organ donation and willingness to donate among health profession personnel.  Nursing students need to have a sufficient knowledge and positive attitude regarding liver transplantation through appropriate education during their course of study.  The aim of the study was to assess Effect of Educational Intervention on Knowledge and Attitude Regarding Liver Transplantation among Nursing Students at College of Nursing Al-Ahsaa. Setting: the study was carried out in on Faculty of Nursing – Al AHsaa. The sample consists of 98 level four nursing students. Tools: Student Self-assessment questionnaire was used pre and post the Educational Intervention for the purpose of data collection to assess knowledge and attitudes of nursing students related to liver transplantation. Research design: Quasi Experimental design was used in this study. Data were collected in Fall semester 2020/2021. The results proved that; student' knowledge has been enhanced and their attitude has been improved after application of the educational intervention, in addition to highly significant correlation between knowledge score of the studied students and their attitude post the intervention, moreover; there was a significant correlation between total knowledge level score with student' previous Information about liver transplantation pre the intervention. Conclusion and recommendation: implementation of the educational program positively affected the nursing students' knowledge and their attitude toward liver transplantation. So, it is recommended to include organ transplantation education in the nursing program to raise the students' awareness and positively change their attitude toward organ donation and transplantation.