Aim of the study: This study aims to assess prevalence and effect of premenstrual syndrome (PMS) among female nursing students at University of Medical Sciences and Technology, Sudan. Methods: A descriptive study was conducted to examine PMS among female nursing students enrolled in the Faculty of Nursing Sciences at the University of Medical Sciences and Technology. The study included eighty-six participants aged between 18 and 24 years old. They were selected using convenience sampling. Data were collected using questionnaires. The data was analyzed by SPSS. Results: This study focused on nursing students at the University of Medical Sciences and Technology, Sudan. It was found that 40.70% of the participants experienced PMS. PMS impacts daily activities, such as eating, recreation, and social interactions to varying degrees. Severe mood swings and nervous tension (15.8% and 13.2%, respectively) were prominent among the PMS-related behaviors. Conclusion: The investigation of nursing students at the University of Medical Sciences and Technology, Sudan, shed light on the demographic composition and prevalence of PMS among nursing students. This study underscored the predominance of female students and reveals that a significant proportion of them experienced PMS. Recommendations: The findings highlighted the urgent need for effective university-based health promotion polices and interventions that focused on orienting students at the university on the PMS and its effect. Further studies should be conducted with more sample on a larger community.