Background: Egg frozen is a crucial component of assisted reproductive technology and can significantly enhance the biology of reproduction and the treatment of infertility. Aim: To evaluate the effect of an educational program on knowledge and behavior about egg frozen among unmarried females. Research design: A quasi experimental pretest/posttest research design was conducted. Sample: A convenience sample includes 300 working unmarried females at Minia University. Tools: data were collected via two tools. tool I: The structured interview   questionnaire and Tool II: behavior assessment tool regarding egg frozen. Results: Unsatisfactory knowledge scores in the pretest for 86% of the investigated unmarried females were lowered to 10% and 13% of them in the post test immediately and follow up, respectively, with a highly statistically significant difference. Additionally, 91.7% and 88.3% of the investigated unmarried working females had unfavorable beliefs about egg freezing in the posttest immediately and follow up, respectively, with a statistically significant difference. In contrast, 90% of them had negative beliefs in the pretest. Additionally, with a statistically significant difference, just 10% of them showed a favorable attitude towards frozen eggs in the pretest. Conclusion: After the execution of the teaching program, there were highly statistically significant improvements in the total knowledge and behavior of unmarried females regarding egg frozen. Recommendation: Delivering health education campaigns regarding frozen eggs through the ministry of health and in cooperation with Egyptian Dar Al Iftaa.