Background: There is a vast growth of Artificial Intelligence (AI) applications across all aspects of healthcare. Nursing practice is critical where AI technology will enhance practice and patient outcomes.The aimof this study was to evaluate the effect of educational program on nurses' knowledge and attitudes regarding artificial intelligence.Design:A quasi-experimental design was used in this study. Setting: This study was conducted atMansoura University Hospitals at medical and surgical departments. Sample: A simple random sample included (203) nurseswere selected from the previously mentioned settings. Tools: Two tools were used to collect the necessary data for achieving the study objective: A self-administered Artificial Intelligence Knowledge Questionnaire and A General Attitudes towards Artificial Intelligence Questionnaire.Results: The primary source of information for nurses' knowledge about artificial intelligence was from Internet (73.9%).The total level of knowledge was satisfactory among 16.3% of studied nurses at pre intervention period, while it was 82.8% at immediately post intervention, and decreased to 68% at follow-up. Additionally, the total positive nurses' attitudesmean score improved from 53.35 ±21.07 at pre-interventionto 77.19±18.64 at immediately post and decreased to 67.95±23.62 at follow-up with statistically significant differences. Moreover, highly statistically significant differences between the demographic characteristics (marital status, and educational qualifications) and total knowledge level post intervention (P<0.001). Conclusion: This study revealed that the educational intervention had highly statistically significant positive effect on the studied nurses' knowledge, and attitudes regarding artificial intelligence. Therefore, it is recommended toencourage nurses to increase their knowledge and attitudes toward artificial intelligence through workshops and training programs, and providing further education and training are required to enable them integrate AI into nursing practices.