Background: Difficulty in passing urine and increased frequency of urination are common symptoms of Benign prostatic hyperplasia (BPH), a prevalent condition among elderly males characterized by the enlargement of the prostate gland, and urinary tract infections. Patients with BPH who require catheterization are at higher risk for developing Catheter-associated urinary tract infections (CAUTIs), which makes such a group of patients in a crucial need to develop effective strategies to prevent and manage such conditions. Aims: This study aimed to evaluate the effect of self-care practice guidelines of Catheter-associated urinary tract infections (CAUTI) on patients with benign prostatic Hyperplasia (BPH). Methods: A quasi-experimental study was conducted in the urology departments at Alexandria Main University Hospital. A convenience sample of 60 adult patients with BPH was equally divided randomly into two groups: a study group (n=30) and a control group (n=30). Two tools were used for data collection: a (BPH) patients' knowledge assessment questionnaire and a (BPH) sign and symptom severity structured interview schedule. Results: The study showed a significant improvement among the study group's knowledge, international prostate symptom score (IPSS) symptom severity, quality of life, catheter care, and patients' practices for urinary tract infection-related signs and symptoms and catheter care, comparing to the control group. Conclusion: The provision of effective education had a profound impact on improving patients' knowledge and practice.