Background: Trauma-informed care includes a range of practices that build a culture of safety, healing, and empowerment. Burns' practices in pediatrics don't fully incorporate trauma-informed care (TIC), as the nature, extent, and impacts of trauma are not understood. Nurses are uniquely positioned to provide trauma-informed care, as they care for children in a serious and complex healthcare setting. The study aimed to evaluate the effect of trauma-informed care educational program on nurses' knowledge and practices at the pediatric burn unit. Study design: A quasi-experimental research design using one group (before, immediately after, and 2 months follow-up) was used. Setting: The study carried out at the Pediatric Burn Unit at the Plastic, Reconstructive, and Burn Surgery Center at Mansoura University Hospitals, Egypt. Subjects:  A convenience sample of (65) pediatric burn nurses were recruited in the study. Tools: Data were collected using nurses' knowledge structured questionnaire and Nurses' Self-rated competence scale. Results: The study revealed that there was a significant improvement in nurses' knowledge. All nurses showed incompetent score levels of practice before intervention. While the majority of them had a competent level of practice immediately and after two months of intervention. There were highly statistically significant differences in relation to most items of nurses' opinions regarding trauma-informed care before and after the educational intervention. Conclusion: Implementing an educational program about trauma-informed care effectively improved nurses' knowledge and practices about the importance of incorporating both medical and psychological care at the pediatric burn unit. Recommendation: Periodic trauma-informed care training for nurses is needed and should aim to build nurses competence in providing trauma-informed care in different health care sitting.