Context: Genitourinary anomalies account for the most common urogenital disorders in children. Therefore, pediatric nursing excellence model will continue to be central to excellence in nursing practice and optimal childcare. Aim: To evaluate the effect pediatric nursing excellence model on nurses' knowledge and practice regarding care of children undergoing surgical repair of genitourinary anomalies. Research design: Researchers utilize a quasi-experimental research. Setting: The present study was conducted at the inpatient pediatric surgical department, at Benha Specialized Pediatric Hospital in Benha city. Study sample: All available nurses, they composed of 60 nurses working at the previously mentioned settings were included in the current study regardless of their personal characteristics. Study tools: Three tools were used for data collection, Tool I: A structured interviewing questionnaire developed to assess nurses' personal characteristics and level of knοwledge regarding care of children undergoing surgical repair of genitourinary anomalies. Tool II: An observational checklist to assess nurses' practice regarding care of children undergoing surgical repair of genitourinary anomalies Tool III: A structured questionnaire based on Pediatric Nursing Excellence (PNE) model regarding care of children undergoing surgical repair of genitourinary anomalies. Results: The majority of the studied nurses had satisfactory level of knowledge and competent level regarding their total practice post PNE model application. Also, the majority of them had high excellence level post PNE model application, while less than three-quarters had low excellence level pre PNE model application. Conclusion: It could be concluded that nurses' level of knowledge and practice improved regarding the care of children undergoing surgical repair of genitourinary anomalies, which emphasize that PNE model application was effective. Recommendation: Developing strategies to improve nursing care for children undergoing surgical repair of genitourinary anomalies is needed to achieve high competent nurses' performance