Fetal cardiotocography is a type of electronic fetal monitoring used to evaluate the health of the foetus and find any alterations that put the fetus at risk. Aim: to evaluate the effect of training program on knowledge and interpretation skills of fetal cardiotocography among undergraduate nursing students Research methods: A quasi-experimental design was employed.  Setting: The study was carried out at the Nursing Faculty of Minia University in Egypt. Sample: Two hundred and ninety-seven (297) students with odd numbers who enrolled in the second semester and studying obstetrics & gynaecology nursing course during the academic year 2021–2022 were selected by a systematic random sampling procedure from the total number of five hundred and ninety-five (595) students. Tools: Three tools were utilized for data collection, tool 1, socio-demographic data, tool II, knowledge about fetal cardiotocography, tool III, interpretation skills regarding cardiotocography. Results: Mean score of total knowledge of students was 19.41± 1.535 in pre-test, increased to 24.27± 2.263 in post-test with statistically significant differences (p-value =.0001).   Additionally, the mean score of interpretation skills of fetal trace was 25.92 ± 2.81 in pertest, increased to 38.18 ± 5.52 in post-test with statistically significant differences (p-value =.0001). Also, there was a strong positive correlation between student's knowledge and their interpretation skills in post-test with statistically significant differences (p-value .003). Conclusion: There were statistically significant differences in knowledge and interpretation skills in pre-test and post-test which indicate a positive effect of the training program among undergraduate nursing students. Recommendations: Providing continuous training programs concerning fetal cardiotocography not only for nursing students but also for staff nurses in maternity hospitals which result in the improvement of the quality of maternal and new-born care.