Aim: This study aimed to explore knowledge, attitude, and practice of female toward Breast Self-Examination (BSE) at Abha city. Research design: A descriptive- cross sectional design was utilized to conduct this study. Setting and sampling:  A convenient sample of 508 female from different primary health care centers at Abha city South-western, Saudi Arabian. Tools of Data collection included a structured interviewing questionnaire to cover data related to socio demographic characteristics, knowledge about breast self-examination, attitude toward breast self-examination and practice toward breast self-examination. It was Google form. Results:  More than half of female didn't perform  breast self-examination in previous twelve months (53.7%) ,less than  half of them had satisfactory level of knowledge (40.4%), one third of them had fair knowledge (34.4%) and fourth of them had unsatisfactory level of knowledge(25.2%). Moreover, more than two thirds of female had negative attitude towards BSE (66.5%).In addition, more than half of studied female had unsatisfactory practice (64%) related to BSE. Conclusion: This study concluded that most of female had unsatisfactory knowledge, more than half of them had unsatisfactory practice and more than two third of them had negative attitude toward breast self-examination. Recommendation: additional effort by health care provider to conduct campaign program in primary health care centers and university to improve awareness of female about breast self-examination.