Background: Oocyte cryopreservation is a unique and significant advanced reproductive technology method for women who want to postpone conception for a particular time to preserve fertility and for those who are receiving chemotherapy. Aim: To investigate the effect of an educational intervention on knowledge, attitude, and beliefs of unmarried, healthy females regarding oocyte cryopreservation. Settings: The study was carried out at Shebin El-Kom, Menoufia University, in the faculties of science, commerce, and art. Design: This study employed quasi-experimental design. Sample: A purposive sample consisting of one hundred healthy and single women was chosen. Instruments: A structured interview questionnaire; unmarried, healthy females' beliefs and attitudes about oocyte cryopreservation. Results: This shows that 89% of the healthy females in the study knew very little about oocyte cryopreservation prior to the intervention, but 88% of them knew a lot about it a month later. Additionally, before establishing the intervention, only 19.0% of the participants had good opinions about oocyte cryopreservation; however, a month later, 95.0% of the individuals had the same beliefs. Furthermore, one month following the intervention, 95% of the participants had positive attitudes concerning oocyte cryopreservation, compared to 92% of those who had negative attitudes prior to the intervention. Conclusion: Implementation of an educational intervention was effective in increasing the studied unmarried, healthy females' knowledge and changing their attitudes and beliefs towards oocyte cryopreservation. Recommendations: A health education intervention regarding oocyte cryopreservation should be provided to all unmarried females of fertile age to help them make an informed decision to preserve their fertility.