Background: Reproductive health of adolescent girls is a top priority in global programs. People with disability need access to health information and services across the life course for the same reasons as people without disability. Aim: To evaluate the effect of educational program utilizing audio and braille text on knowledge and practice of visually impaired adolescent girls regarding reproductive health. Methods: A quasi-experimental design (one group pre-posttest) was utilized. Sample: A purposive sample of ninety visually impaired adolescent girls between the ages of 11 and 18, who were willing to participate in the study, was recruited for the purpose of the current study. Three tools were used for data collection: Tool I, Visually Impaired Adolescent Girls Structured interview questionnaire. Tool II, visually impaired adolescent girls' knowledge assessment questionnaire related to reproductive health. Tool III, visually impaired adolescent girls' reported practices related to reproductive health. Results: After implementation of the educational program, all the studied visually impaired girls (100%) and (96.7%) of them had a satisfactory level of knowledge regarding reproductive health immediate posttest and follow-up respectively, compared to (41.1%) only in the pretest.  Regarding the total practice scores, 31.1% of the girls only demonstrated appropriate practices in the pretest, increased to 94.4% in the immediate posttest and reached to 100% in the follow-up. A statistically significant relation was found between the levels of knowledge and practice during the pretest, immediate posttest, and follow-up with (p=0.0001). Conclusion: The educational program significantly improved the knowledge and practices among the visually impaired girls regarding reproductive health. Recommendations: Similar educational programs should be implemented in visually impaired students' schools to enhance their knowledge and practices that related to reproductive health. Encourage collaboration between school nurses, and parents to provide comprehensive support for visually impaired girls in addressing their unique reproductive health needs.