Background: Type I diabetes (T1D) is a chronic condition that impacts all part of a patient's life. As a result, stress and fear of hypoglycemia (FOH) can have a severe impact on a patient's mental, physical, and psychological health. Aim: was to evaluate effect of educational program on mothers' knowledge, stress and fear of hypoglycemia and their children glycemic control Research design: A quasi- experimental research design was used in this study. Sample and Setting: was conducted on one hundred mothers who have been selected for the program (pre and post) in pediatric department at General and Health Insurance Hospitals. Tools of data collection: four tools for data collection were used tool (1) An interviewing questionnaire sheet, (2) Pediatric Inventory for Parents (PIP), (3) The Hypoglycemia Fear Survey–Parent (HFS-P) (4) Glycemic control. Results of this study showed that the mean age of mothers was 33.5 ± 20.1,  and noticeable improvement in the mothers' knowledge about T1D  in post-test than in pre-test, the total mean scores of Pediatric Inventory for Parents (PIP) and  The Hypoglycemia Fear Survey–Parent (HFS-P) were alleviated in post-test than in pre-test. while  Glycemic control  of school age children were better controlled in post-test at 3 and 6 months than in pre-test. So, this study concluded that improving mother's knowledge about T1D, alleviating mothers stress, fear of hypoglycemia, after education program and the children's glycemic control improved after health education program. This research recommended that Future studies are needed to create and improve interventions through educational programs in alleviating stress, fear of hypoglycemia for mothers taking care of children with T1D.