Back ground: Street cleaners play important roles in environmental health by keeping roads clean; nonetheless, these workers confront several hazards. The majority of these dangers are avoidable. Aim of the study: Preventive health program regarding knowledge and practice of street cleaners about occupational health hazards in Sohag city. Subjects and Methods: A quiz -experimental research design was utilized in this study. The study was conducted in Sohag Local Council at Sohag city Sample: Total coverage 208 street cleaner workers in Sohag Local Council Convenient sample was used for workers. The total number was 208 workers: Two tools were used in this study; the first tool was a structure interview questionnaire sheet about Socio-demographic characteristics and worker's knowledge about occupational hazards, prevention of health hazards. The second tool was observational checklist was used to evaluate protective measures of street cleaner workers. Results: the majority of the street cleanes were poor knowledge and practice about occupational hazards and prevention of health hazards before implement of the program. respectively. However, there was significant improvement in knowledge and practice of the street cleaners after implementation of the program. The total of knowledge and practice were statistically significant (p<0.001). Conclusion: The implemented program about prevention of occupational hazards had a good impact on street cleaners' knowledge and practice. Recommendations: personal protective devices should be available for all street sweeper workers.