Background: Carpal tunnel syndrome has a great impact on the pregnant woman's daily life. Several non-pharmacological techniques have been used to alleviate these symptoms. Aim: The present study aimed to examine the effect of nursing instructional program on neurophysiologic parameters of carpal tunnel syndrome among pregnant women. Methods: A quasi-experimental research design was adopted. A convenient sample of 100 pregnant women with carpal tunnel syndrome was enrolled. This study was carried out at the obstetric and gynecological outpatient clinic of Kafrelsheikh general hospital, Kafrelsheikh governorate, Egypt. Three tools were used to collect data: A structured interviewing Questionnaire, Numeric Pain Rating Scale, and Boston Carpal Tunnel Syndrome Questionnaire. Results: The mean wrist pain scores at baseline assessment didn't show a significant difference between the control and intervention groups (p>0.05). However, post-intervention, the difference in mean wrist pain scores between the two groups was highly statistically significant (p<0.001). When comparing the symptom severity scale and functional status scale between the two groups, the differences weren't statistically significant at baseline assessment (p>0.05). Post-intervention, the difference in symptom severity scale was highly statistically significant (p<0.001) between both groups while the functional status scale did not show a significant difference (p>0.05). Conclusion: The nursing instructional program was successful in improving the neurophysiologic parameters of carpal tunnel syndrome among pregnant women. Recommendation: Applying such nursing instructional program should be enforced to decrease suffering among pregnant women with carpal tunnel syndrome.