Background: Kangaroo mother care (KMC) is a standardized and protocolized care system for low birth weight, based on skin-to-skin contact between the infant and the mother. The mother's active participation is crucial for effectively implementing kangaroo mother care services to improve physiological stress parameters for neonates. Aim: Evaluate the effect of educational discharge guide program for awareness toward kangroo mother care on mothers and preterm neonates' outcomes. Design: Quasi experimental research study design was used. Setting: The study was conducted at Postpartum Unit in Women's Health Hospital Assuit University. Sample: A purposive sample of 100 mothers of low birth weight neonates. Tools: Data collection tools included five tools: structured interviewing questionnaire, mothers' knowledge assessment questionnaires, attitude assessment questionnaires, mothers' observational checklist, and physiological stress parameters sheet of preterm neonates. Results: The result of this study revealed that there was highly statistical significance difference in the total means score of knowledge, practice and attitude after receiving kangaroo mother care discharge guide program with p-value 0.001. Additionally, kangaroo mother care showed more stability in physiological stress parameters among preterm neonates. Conclusion: The findings of this study support the research hypotheses that mothers who received KMC discharge guide program showed a positive effect on mother's level of  knowledge, attitude, practice and maintaining preterm neonates' physiological stress parameters as compared to before intervention.  Recommendations: The neonatal nurse's should apply the KMC discharge guide program to enhance mothers` knowledge, attitude and practice to maintain preterm neonates' physiological stress parameters especially after discharge. Also, work with policymakers and health administrators to develop institution-specific guidelines for KMC implementation and standard operating procedures.