Background: Premature neonates are vulnerable humans requiring much care and attention. pain experience in neonatal period leads to short- and long-term complications that could be prevented by means of neonatal pain relief. Aim: To assess the effect of facilitated tucking position on premature outcomes and pain intensity induced by invasive procedure in premature. Methods: Researcher conducted a quasi-experimental research design at neonatal intensive care units at Benha Children's Specialized Hospital. A purposive sample of premature neonates were randomly divided into two groups: control and study, each with 34 premature neonates. Researcher collected the data through neonatal characteristics, Vital signs sheet, Premature neonates pain profile, neonatal behavioral state. Results: The study revealed that 64.4% and 35.6% of study group had mild and moderate pain, while 48.9% and 8.9% of control group had moderate and sever pain post invasive procedure. Also, mean of quiet sleep of premature neonates in study group was 16.03±3.78, while in control group was 7.33±2.92, at p value 0.005**. Conclusion: Facilitated tucking position had significantly decreasing the severity of pain compared to control group. Also, the current results mentioned that, less than two thirds of study premature neonates had mild pain, while about half of them at control group suffered from moderate pain. In addition, facilitated tucking position decrease change in heart rate and oxygen saturation. Moreover, increase time of quiet and active sleep, while decrease time of active alert and crying among premature neonates Recommendation: Facilitated tucking position should be a part of the routine care for all premature admitted to NICUs. An illustrated leaflet demonstrating step by step about facilitated tucking position should be available at neonatal intensive care units. Continuous training program about facilitated tucking position should implement regularly.