Background: Urogenital tract infections (UGTIs) are a universal public health problem, mainly neglected by many females in adolescence stage. The study aimed to: Assess the prevention of urogenital tract infections among nursing and non nursing female school students. Research design: A descriptive design was utilized to achieve the aim of this study. Setting: The study was conducted at the Technical Nursing Institute in Bab El-sharia University Hospital Affiliated to ElAzhar University, Egypt and secondary school for girls in Bab El-sharia affiliated to Bab Al- Sharia Educational Administration. Sample: A convenience sample of 304 (196 Female nursing school students and 108 of non nursing school students) enrolled at first and second grade at the above mentioned setting was involved in this study. Tools: Self-administered questionnaire: It was included five parts as following: Part (1): female nursing and non nursing school student's demographic data. Part (2): female's nursing and non nursing school student's health history related to exposure to urogenital tract infections. Part (3): females nursing and non nursing school students Knowledge related to urogenital tract infections. Part (4): female nursing and non nursing school students reported Health practices regarding prevention of UGTIs. Part (5): assessing the health needs and problems of female students during infection with the genitourinary system Results: Findings of the present study showed that 50% of the studied nursing students, had satisfactory level of total knowledge about urogenital infection, compared with 34.3% of the studied non nursing students had satisfactory level of total knowledge about urogenital infection. 58.2% of the studied nursing students had unhealthy practices to prevent urogenital tract infection compared with 61.1% of the studied non nursing students had unhealthy practices to prevent urogenital tract infection. Conclusion there was a statistically non-significant correlation between total practices and age. Also, there was a statistically non-significant correlation between total practices and total knowledge of the studied students (nursing and non- nursing). Recommendation: Students counsel about personal hygiene and importance of menstrual hygiene is recommended. Further study: Designing and implementing workshops about proper hygienic practices, healthy eating habits would be of great importance to increase students' awareness and prevention of UGTI