Background: Leadership practices and knowledge sharing are major factors for the success of organizations. Aim: The aim of the study was to assess the relationship between staff nurses perception regarding nursing managers` leadership practices and their knowledge sharing. Subjects and Methods: A descriptive correlational design was used in carrying out this study. The study was conducted at El Sheikh Zayed Al Nahyan Hospital. Study subjects were 129 out of 190 staff nurse. Tools: Data collection tools were Leadership Practices Inventory and Knowledge Sharing questionnaire. Results: Staff nurses' perceived their nurses managers' leadership practices were high frequency as less than three quarters of them had high perception level toward total leadership practices. Also, staff nurses had high level of knowledge sharing and they had high level of individual knowledge sharing and organizational knowledge sharing. Conclusion: There was positive statistical significant correlation between nurse managers' leadership practices and staff nurses knowledge sharing. Recommendations: Leaders need to facilitate knowledge sharing by building positive work environment and listen to the dreams of nurses for creating trust and emotional relationships with them to enhance their knowledge sharing and builds.