Background: Spiritual leadership is a sense of spiritual well-being that positively affects organizational outcomes such as enjoyment of work which associated with enhancing work attitudes, adaptability, and performance. Aim of the study: Assess spiritual leadership educational program for head nurses and its effect on staff nurses' job enjoyment. Research Design: A quasi- experimental design was used to achieve the aim of the present study. A quasi-experimental is an empirical interventional study used to estimate the causal effect of an intervention on target population without random assignment. Setting: The study was conducted at Benha University Hospital in all departments and units. Sample: A convenient sample of 35 head nurses, and 320 out from 1600 staff nurses. Tools of data collection: Data of the present study was collected by using three tools , spiritual leadership knowledge questionnaire, spiritual leadership skills questionnaire and job enjoyment questionnaire Results: Minority (6.3%) of studied head nurses had high knowledge level at the preprogram phase while more than three quarters 82.5% & 79.3% at post program and follow up study phases respectively, less than one quarter 16.3% of them had high spiritual leadership skills level at preprogram phase while more than three quarters (85.5 % & 81.3%) of them at post program and follow up phases respectively. Minatory (6.0%) of the studied staff nurses had a high enjoyment level at preprogram phase while near to three quarters (69. 4%: %) of them had a high enjoyment level at the post program and follow up phases respectively. Conclusion: It was concluded that spiritual leadership had positively statistically significant correlated with job enjoyment. Recommendation: Hospital administration should conduct a training program for different levels of nurse managers to promote spiritual leadership practices in their organization