Background: Cardiopulmonary resuscitation (CPR) is a basic emergency procedure consisting of manual artificial respiration and external cardiac massage. Good quality CPR that was initiated early by witnesses increased survival after cardiac arrest from 3.9 % to 16.1%. So, it is essential to train teachers and students to increase the proportion of qualified individuals in society. Objective: To Investigate the effect of cardiopulmonary resuscitation training on the knowledge and skills of high and middle schools `s students and teachers. Setting: The study was conducted at forty middle and secondary female schools from the East educational office in Jeddah, Saudi Arabia. Subjects: A convenient sample of 45 female teachers who were nominated to act as student`s guide or health guide and 45 students who were members of the school health association affiliated with previously selected schools. Tools: Two tools were used. Tool one: Pre/Post knowledge questionnaire, Tool two: CPR observational checklist.  Quazi experimental design was utilized to conduct the study. Result: Most of the participants (75.6%) had a previous CPR background, and 87.8% of them recommended adding CPR training to the school curricula. A significant statistical difference was found with the mean knowledge score while 84.4% had good CPR skills post-training. However, no correlation was observed between knowledge and skills scores. Conclusion: The level of knowledge and CPR skills were improved among the study participants after the training as well they recommend adding CPR training to the school curriculum. It reflects the need for continued training that may play an important role in saving more lives and disseminating CPR essential knowledge to the community. Recommendation: Include CPR training as an essential component in the school curriculum.