Introduction: Chronic wounds represent a significant public health challenge, particularly for older individuals with comorbidities. This research aims to explore the potential of nanotechnology developments in the biomedical field, specifically investigating the use of polyurethane nanofiber embedded with a combination of Zinc Oxide (ZnO) and Graphene Oxide (GO) for wound healing applications. Methods: the study utilized the electrospinning method to fabricate the polyurethane nanofibers, and a green synthesis approach of zinc oxide. The wound healing properties of the ZnO-GO-embedded polyurethane nanofibers were evaluated through a series of characterization techniques, including cell migration assays, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Conclusion: The findings of this research suggest that the utilization of polyurethane nanofibers could be a viable approach for addressing the challenges associated with chronic wound management.