he objectives of this study was to identify the additives present in commercial Henna pastes in Jazan market, and to investigate if it contain harmful materials as paraphenylenediamine (PPD). The pH value of henna samples was found comparable except for some of the pastes that may prove harmful effect on use. Henna samples were found to contain both alkaloids, resins, flavonoids, and tannins . It does not contain saponines. The FTIR spectral analysis of Henna samples demonstrated characteristic absorptions of OH, C = O, C-H, and C = C, while NH peak was detected only for Jazan sample. This sample was found to contain 19% PPD content. The GC-MS analysis revealed also the difference of this sample on comparing with pure Henna powder. A natural substituent is suggested that give henna pastes dark color, and safe for use.