Background: Obstructive sleep apnea (OSA) is a widespread health issue increasingly linked to cardiovascular complications, including dysfunction of both the left ventricle (LV) and right ventricle (RV), arterial hypertension, coronary artery disease, as well as heart failure, and the risk of arrhythmias. Objectives: This study aimed to assess the impact of obstructive sleep apnea (OSA) on myocardial mechanics utilizing Two-dimensional speckle tracking echocardiography (2D-STE). Subjects and methods: 45 patients diagnosed with OSA through polysomnography were set to be classified into three groups with respect to their apnea-hypopnea index (AHI): Mild, moderate and severe. Additionally, a control group comprising 45 healthy individuals carefully matched for sex and age was included. All participants underwent a thorough echocardiographic evaluation, which included both conventional 2D echocardiography and 2D-STE. Results: While conventional 2D echocardiography did not reveal significant differences in LV dimensions and function among the study groups, 2D-STE analysis indicated statistically significant variations in LV and RV strain values. The most significant impairments were detected in the severe as well as the moderate OSA groups when compared to the other mild OSA and control groups. Conclusion: Our findings suggest that OSA adversely affects both LV and RV myocardial mechanics. The severity of these impairments correlates with the severity of OSA. By employing 2D-STE to assess LV and RV strain, clinicians can identify subtle cardiac alterations in OSA patients before traditional echocardiographic measures or the onset of heart failure. Early detection enables timely intervention and management strategies to address OSA-related cardiac complications.