Objectives: Elevation of TGs levels has been involved in the pathogenesis of cardiovascular disease (CVD), so the regular assessment of its level is very important. Objective: This study aimed to compare the levels of TGs in fasting and non-fasting situation in same individual. Methods: In one hundred normal subjects, TGs levels were compared in fasting and non-fasting status in relation to sex, age, BMI, smoking, duration of fasting, and fat content in last meal. Results: The only independent predictor for high non-fasting TGs level was the fat content in the last meal (p: 0.003). Conclusion: High fat content in the last meal significantly elevates TGs level in comparison with fasting level in same individual.