Background: Among the most common medical conditions affecting middle-aged men, erectile dysfunction (ED) can have an impact on the well-being of the patient and his partner. Several medical specialties have made use of platelet-rich plasma (PRP) for decades. Objective: To evaluation of the efficacy of platelet-rich plasma (PRP) cavernosal injections in the management of erectile dysfunction, and to study its safety and adverse effect in the treatment of erectile dysfunction. Subjects and methods; This intervention clinical prospective study was conducted at multicenter including Badr University Hospital and AL Haram Hospital on 40 patients suffering from erectile dysfunction, using PRP cavernosal injection. Follow up of erectile function and penile hemodynamics involved evaluation of patients utilizing International Index of Erectile Function (IIEF-5) at 3, 6 months after initiation of therapy together with PCDUS. Result: The mean ED duration was 18.62±4.9-month. There were 16 patients with mild ED (40%), 18 patients had mild to moderate ED (45%) and six patients had moderate ED severity (15%) according to IIEF grading. The mean IIEF-EF pre-treatment was 15.35±3.1, the mean IIEF-EF after 3^rd month was 17.28±2.95, and the mean IIEF-EF after 6^th month was 19.40±2.74, regarding follow up of IIEF-EF of the study population. Statistical analysis revealed that the groups under consideration differed significantly with respect to the IIEF-EF at baseline, the third month, and the sixth month. Conclusion; A negative significant correlation was revealed between percentage of change and ED duration, which means less improvement in patients with longer duration of erectile dysfunction.