Background: Portal vein thrombosis (PVT) is a recognized complication of liver cirrhosis. The pathogenesis of PVT is multifactorial. The extent to which thrombophilia promotes PVT and whether patients with PVT should be routinely tested for thrombophilia remain uncertain. Objective: This retrospective research aimed to assess the frequency of thrombophilia in cirrhotic patients with non-malignant PVT to clarify the relevance of thrombophilia testing in this particular context. Patients and methods: This study involved 40 cirrhotic patients with protein C & S deficiency prior to liver transplantation, where 20 patients had PVT and the other group (20) had no PVT. Thrombophilia testing included gene analysis for beta-fibrinogen -455G>A, factor XIII V34L, factor V H1299R (R2), prothrombin G20210A, factor V Leiden, methylene tetrahydrofolate reductase (MTHFR) C677T, and MTHFR A1298C. Results: Regarding the thrombophilia gene screen, no statistically significant differences were detected between the two groups. In addition, the inherited thrombophilia gene status was not a risk factor for PVT. However, factor V Leiden showed a high odds ratio of 11.457 and 95% CI of 0.885 - 148.27. Conclusion: Inherited thrombophilia is considered to be a low-risk factor with low prevalence in patients with cirrhotic non-malignant PVT. Therefore, routine testing for these conditions does not seem to be indicated in this setting.