Background: There is a lack of extensive study investigating the possible connection between vitamin D levels and glucose metabolism in children undergoing growth hormone (GH) treatment. Aim: This research aims to examine the alterations in vitamin D and fasting glucose levels after one year of GH therapy in youngsters. Methods: A longitudinal cohort research tracked a group of 40 youngsters (aged 8-14 years) who required growth hormone (GH) medication due to medical reasons. Prior to commencing treatment, baseline evaluations were performed to measure the levels of vitamin D (in ng/dL) and fasting glucose (in mg/dL). Subsequent evaluations were conducted after one year of uninterrupted growth hormone therapy. Results: Growth hormone therapy decreased vitamin D levels and elevated fasting glucose levels, potentially impacting blood sugar control and insulin sensitivity. Insufficient vitamin D levels can lead to health issues. Conclusion: GH medication affected vitamin D levels and possibly affected glucose homeostasis. These data might contribute to future studies on the impact of vitamin D supplementation in enhancing growth and metabolic well-being in children undergoing GH therapy. Further research is needed to determine the full impact of growth hormone therapy on individual health and other factors.