Background: Estrogen, which is usually associated with women, is important for a variety of physiological activities in men, involving metabolism of bone, testicular, cardiovascular, & sexual function. Aim and objective: This study aimed to assess the correlation among estrogen receptor gene 1 Pavol Ivanyi (Pvu) П polymorphism & male infertility. Patients and methods:  This Cross-sectional comparative research was performed on 92 Egyptian married men who were divided into 2 groups. Results: There was a positive association among single nucleotide polymorphisms (SNPs) results & motility, vitality, and abnormal sperm forms in the fertile group (p < 0.001, <0.001 & p= 0.008 respectively), but no significant alteration among SNP results and count or volume (p=0.416 & 0.087 respectively). There was a positive association among SNP result & sperm count (p=0.006), while there was no significant variance among SNP result & semen volume, total sperm motility, vitality and abnormal forms in infertile group (p= 0.512, 0.070, 0.145 & 0.676 respectively). Conclusion: The distributions of Pvull genotype were not significantly various among infertile & fertile groups. However, there was positive correlation between SNP result and motility, vitality and abnormal sperm forms in fertile group. While in infertile group there was positive association among SNP result & sperm count where total sperm count (TSC) genotype was higher than total testosterone (TT) and clomiphene citrate (CC) genotypes.