Background: The Coxsackie virus and Adenovirus Receptor (CAR) is a cellular protein that has a role in cell adhesion, signaling, and viral infection. There is much disagreement over the significance of CAR expression in colorectal carcinoma development, with some research suggesting CAR downregulation and others indicating that CAR enabled complicated effects during colorectal carcinogenesis. Objective: This study aimed to elucidate the difference in CAR expression levels in colorectal cancer (CRC) tissue versus normal colon tissue, and to correlate the expression levels with the disease stage. Patients and methods: Fifty patients with proven colorectal cancer were enrolled in this study. During surgical excision treatment, 50 pairs of CRC tissue and normal tissue samples were obtained and examined for CAR expression levels using reverse transcriptase Real time PCR. Results: CRC specimens showed significantly downregulated CAR gene expression when compared to nearby safety margin specimens. No significant differences were found in CAR gene expression levels in CRC tissue based on patients' gender, tumor site, size, associated LN metastasis and tumor stage (p > 0.05 for each). However, stratifying cases into early (stages I and II) and advanced (stages III and IV) revealed that lower CAR gene expression was significantly associated with advanced CRC stages. Conclusion: Low CAR gene expression may have a potential role in colorectal carcinogenesis and its level is associated with advanced CRC stages with poorer prognosis.