Background: Vitiligo manifests as depigmented dermal patches developed owing to the loss of melanocytes. It has a major undesirable effect on sexual activity owing to the deforming skin lesions affecting self-image and self-esteem. Sexual dysfunction is common and may influence 30–70% of women, leading to individual trouble. Objective: This study aimed to assess the sexual function and dysfunction in females with vitiligo, and to evaluate the quality of life (QOL) in relation to disease severity. Patients and methods: This study was a prospective case-control study, comprising 100 females with vitiligo in the childbearing period (patient group) and 100 healthy volunteers (control group). Evaluation of vitiligo activity was done via Vitiligo Disease Activity (VIDA) score & Vitiligo Area Scoring Index (VASI). Patients and controls were asked to complete the Arabic validated version of Female Sexual Function Index (ArFSFI) questionnaire & Dermatology Life Quality Index (DLQI). Results: Total FSFI average score was significantly lower in cases than control with 65% defective pattern in cases, which was significantly higher than control (22%). Slight difference between patients with VIDA (0 &1) and those with VIDA (2, 3 & 4), as regards all items and total average scores of FSFI scale. However, the differences were statistically non-significant. Slight difference between patients with no or slow response to treatment and those with satisfactory response as regards the total average scores of FSFI scale Conclusion: According to our study, patients with vitiligo were demonstrated to be at greater risk of sexual dysfunction.