Background: In the elderly stomach, Helicobacter pylori (H. pylori) is a highly common harmful organism. It raises the chance of gastric cancer and atrophic gastritis. High incidence and medication resistance are two characteristics of infection with H. Pylori in older people.  Objective: The study intended to investigate the relationship between infection with Helicobacter pylori in the elderly Egyptian population and ABO blood groups. Subjects and methods: 600 people who were 65 years of age or older, with any symptoms of dyspepsia, bloating, nausea and vomiting, burping, and loss of appetite, participated in the study. Patients were referred for stool antigen testing to diagnose H. pylori infection. Participants were in two groups Group I: positive stool antigen patients for helicobacter pylori. Group II: negative stool antigen subjects for helicobacter pylori. Results: The prevalence of H. pylori infection in group Iwas 40.3% in the A blood group, 27% in the B group, 21.7% in the AB group, and 11% in the O blood group. On the other side group II has a different ABO group rate, it was 36.7% in blood group A, 22.7% in group B, 29% in group AB, and 11.7% in group O. Rh factor was positive 76.7% in Group I, and 69.3% in group II. Rh was negative in 23.3% of Group I and 30.3% of Group II. Conclusion: Infection with H. pylori in the elderly Egyptian population currently is affected by the Rh factor and not by the patient's ABO blood group.