Using an indomethacin (IND)-induced gastric ulcer model in rats, we sought to identify the phytochemical components of Bidens pilosa (B. pilosa) leaves and examine their gastroprotective effects. There was a total of 56 male albino adult rats used, split into 7 groups (n=8 rats per group). Group (1) was control -ve normal (non- treated) and group (2) was +ve control given orally 30 mg/ kg indomethacin (IND). GROUP (3) B. pilosa extract I + IND and will receive B. pilosa extract (75 mg/kg b.wt /day) orally.  Groups (4), (5) and (6) were administered IND plus 75, 150 and 300 mg/kg of B. pilosa extract, respectively. Group (7) was given 30 mg/ kg IND and received Ranitidine as a reference drug (50 mg/kg b.wt /day) orally for 2 2 consecutive weeks before IND administration. The experiment concluded with the sacrifice of the rats, the opening of their stomachs, and the collection of blood samples for biochemical examination. Gastric mucosal homogenates were examined for antioxidant enzyme activities. The results showed that dried powder of B. pilosa leaves contains carbohydrates, glycosides, sterols, triterpenoids, flavonoids, tannins, saponins and alkaloids, while Anthraquinones were absent. B. pilosa extract increase body weight gain, increased levels of antioxidant enzymes glutathione peroxidase (GPx), Superoxide dismutase (SOD) & Catalase (CAT) in gastric homogenate and decreased serum liver enzymes. B. pilosa extract alleviated gastric ulceration in rats. Therefore B. pilosa may be beneficial for patients suffering from gastric ulcer. Keywords: