Background: Sepsis is defined as a life threatening organ dysfunction caused by a dysregulated host response to infection, and it is one of the most deadly and costly conditions at ho­spitals. Objective: The aim of the current study was to decrease the incidence of sepsis and mortality related to it among ICU patients through applying educational programs and assessment of physicians' and nurses' knowledge and performance. Patients and methods: A quasi-experimental study was carried out on physicians and nurses in Zagazig University Hospitals during the period between September 1, 2021 and February 28, 2022. The investigated units were the Emergency ICU (EICU) and the Surgical ICU (SICU) at Zagazig University Hospitals. Results: There was significant difference in sepsis and infection control scores pre and post educational program among physicians and nurses, in relation to department where total sepsis and infection control score was statistically higher among Surgical ICU physicians and nurses than Emergency ICU physicians and nurses. Conclusion: Implementation of an educational program has a positive effect on knowledge and performance of ICU physicians and nurses in management of sepsis. Therefore, this may help proper management of cases and reducing mortality rates. Education workshops should be based on the most recent guidelines.