Background: Because theophylline metabolism levels are lower in patients with the A allele at site _2964 (G/A) in the CYP1A2 gene, theophylline should be used with caution for asthmatic children. Aim: To analyze polymorphism of in asthmatic children, the CYP 1A2 gene is involved in theophylline metabolism. Patients and methods: This study was conducted at Zagazig University Hospitals' Allergy Disease Unit, Department of Pediatrics in the period from October 2019 to March 2021. 34 children diagnosed as asthma according to GINA 2019 from 6 to 12 years old were included in this study. All asthmatic patients underwent pulmonary function tests, serum IgE levels, and CYP 450 genotyping using polymerase chain reaction with restriction fragment length polymorphism (RFLP). Results: Regarding gene polymorphism, AA occurred in 14.7%, GA in 26.5% while GG in 58.8% of the studied patients. G allele occurred in 72.1% of patients. There was a statistically significant association between 2964 (G/A) in the CYP1A2 gene polymorphism both genotype and allele and level of asthma control (non with AA genotype had any degree of control). Conclusion:  The -2964 G/A polymorphism was found to be significantly related to increased theophylline clearance, and this polymorphism may influence CYP1A2 enzyme inducibility in Egyptian children with asthma.