Background: According to the data, AgNPs are being utilized more frequently for industrial, consumer, medical, food, and health care reasons as well as for other purposes due to their unique physical and chemical characteristics. Despite NPs' benefits, a variety of issues have increased the risk of toxicity in both people and animals. Objective: main objective of this study is to evaluate the effects of AgNPs produced using a green technique from plant extract (Borago officinalis) on the reproductive system (testis). Methods: Borago officinalis flower extracts are used as reducing agents and stabilizing agents in the production of silver nanoparticles, which is an environmentally beneficial process. 40 adult male rats are separated into 4 equally sized groups. The groups were given distilled water treatment, oral plant extract (5 mg/kg), and intraperitoneal injections of AgNPs 30, 50 mg/kg After 35 days, we evaluated the changes in the tissue of the testis. How were the four groups divided? Results: The results showed that AgNPs significantly increased MDA levels in animal groups treated with various AgNP concentrations as compared to control groups treated only with Borage extract. Additionally, Testosterone levels in animals treated with Borage extract decreased non-significantly as compared to the control group, which experienced a significant decline in testosterone when treated with various AgNP concentrations. Conclusion:  despite being made from plant extract, AgNPs have a detrimental effect on the male reproductive system by changing the testicular functions, particularly the production of testosterone, in contrast to the benign nature of the crude Borage flower extract.