Background: In spite of being invasive and expensive, endoscopic and histologic evaluation measures remain the gold standard for diagnosis of ulcerative colitis. There is a need for widely available, reasonably priced biomarkers for testing outside of endoscopic evaluation. Objective: To evaluate fecal calgranulin C, Neutrophil / Lymphocyte ratio and Lymphocyte / Monocyte ratio in ulcerative colitis patients as noninvasive biomarkers of disease activity and severity compared with colonoscopy. Patients and Methods: A cross - sectional study was conducted on 50 patients with ulcerative colitis and were classified into two groups: Group I: (50) patients in active state and Group II: the same (50) patients in remission state. Patients were subjected to thorough clinical examination, laboratory investigations including fecal calgranulin C and colonoscopic assessment. Results: Fecal calgranulin C, neutrophils, monocytes and N/LR were reliable indicators of activity and severity of active UC compared to inactive UC (p < 0.001). The mean fecal calgranulin C level for UC in exacerbation and remission was (709.30 ± 172.31 and 84.86 ± 19.42) pg/ml respectively. The optimal cutoff was estimated at 185pg/ml with sensitivity and a specificity of 94% and 92%, respectively. Significant elevation of NLR was observed in active UC group compared to inactive UC (2.44 ± 0.56 and 1.56 ± 0.36) respectively. Conclusion Fecal calgranulin C and NLR could be used as noninvasive markers to predict activity and severity of UC and to reduce the need for invasive endoscopies.