Background: A significant section of the population is affected by the common and long-lasting consequence of acne vulgaris called acne scarring. It is essential to create a plan for each instance individually since treating acne scars can be difficult. Objectives: This study aimed to evaluate the result of using subcision with blunt cannula in combination with injection of platelet-rich plasma and micro needling in treatment of post acne rolling scars. Patients and methods: During September 2019 up to January 2021, a pilot clinical trial was conducted on 50 patients suffered from mild to severe rolling acne scars on their face at Menoufia University Hospitals. Results: Ordinary acne and number of sessions for scar types were significantly increased with sub-cession, derma pen and plasma group (69%) than in sub-cession (0%) and in sub-cession and plasma (4%) groups (p < 0.05). While there was a significant difference between the studied groups regarding types of scars where rolling scar was the most frequent among sub-cession and sub-cession and plasma groups than in sub-cession, derma pen and plasma group (p < 0.05). On the other hand, there was no significant difference between the studied groups regarding age of onset of acne, (p>0.05), previous types of scars, (p>0.05) and patient and physician satisfaction, (p>0.05). Conclusion: According to the findings of our study, platelet-rich plasma (PRP) and radiofrequency (RF) microneedling were effective treatments for atrophic acne scars in more than 80% of patients, with few adverse effects.