Background: P. mirabilis possesses multiple virulence factors that enable them to cause infection in various anatomical sites of the human body, including endotoxin, and some of them are secreted into a medium such as the enzyme that analyzes urea, hemolytic enzyme, protease, biofilm, DNase, and others. Excessive use of antibiotics has led to the emergence of multidrug resistance and multidrug resistance bacteria that cause serious infections that are difficult to treat. Objectives: The study aims to molecular investigation of the virulence genes in Proteus mirabilis multidrug resistant. Methods: A total of 100 samples collected from different clinical sources (urinary tract infections, wounds, stool) in sterile containers in different hospitals in Baquba city (Baquba Teaching Hospital and Al-Batool Hospital) were subjected to antibiotic susceptibility study using disc-diffusion method. Molecular detection of virulence genes using polymerase chain reaction (PCR) analysis and amplicons sequenced. Results: The results showed that all isolates had multiple resistance against antibiotic, and the highest resistance was against antibiotic Trimethoprim and lowest resistance towards two antibiotic Imipenem and Amikacin . PCR analysis revealed the presence of flaA ,fliC, mrp < /em>A and rsbA were present in all isolates in a ratio 100 %. Conclusion: Isolates of Proteus mirabilis bacteria, which have virulence factors, it causes many diseases and susceptible to infection through their ability to move, transfer, adhere, and form a biofilm.