Background The difficulty encountered during the imaging manoeuvring is to keep both ends of the C-arm well covered with sterile drapes without disturbing the integrity of the surgical field and de-sterilizing the surgeon or the instruments. The C- arm drape contamination may carry additional source of surgical site contamination and infection. Patients and Methods: Forty-six orthopedic operations were carried out at Orthopedic referral major hospital with the use of C-arm, swabs were collected from C-arm draping preoperatively and postoperatively to evaluate C-arm draping contamination. This was correlated with operations time, traffic in the operating room, door opening frequency during surgery  and C-arm Lateral position frequency. The level of contamination was determined according to type of species isolated. Result: Both C- arm tube and intensifier were sterile before almost all operations, but C- arm tube became contaminated postoperatively in 87 % of operations performed. The most isolated species were Coagulase Negative Staphylococcus (CONS) . The mean time of the operation, lateral frequency of C- arm, person number within operation room, and door opening frequency were higher in one species and two species groups than no growth group (>100 minutes, p=0.002), (>7 times, p <0.001), (> 5 persons/hr, p <0.001), and (>10 door openings, p <0.001) respectively. Conclusion: From the present study, we concluded that C-arm drapes carries an inherent contamination risk that can spread to an operational area; in particular, with long duration of the operation, frequently lateral positioning of c-arm, presence of a greater number of personnel in the operating room and high door opening frequency during the surgery.