Background: Pseudomonas aeruginosa is a commonly known opportunistic bacteria that can produce a wide range of biologically active compounds. The ability of certain strains to generate extra pigments such as yellow pyoverdin, dark red pyorubin, and pyomelanin (dark black), as well as the two primary dyes, were pyocyanin (blue-green) and fluorescein (yellow-green). Aims: To investigate the frequency of some virulence factors genes (phz M, phz S ), of carbapenem resistance P. aeruginosa local isolates. Methods: Clinical samples were taken from wounds, burns, urine, nose, and ear swabs, and 110 isolates of Pseudomonas aeruginosa were obtained from two hundred and ten samples gathered from affected patients, and diffusion method was utilized to assess the resistance of the bacterial isolates.  Also was used molecular detection of ( Phz M, Phz S) genes by using PCR technique. Results: Most of the isolates (40) % were found in burn samples whereas in wounds samples (16.3)%,  urine (23.7)%, and ear swabs (20)%. In the present study, a prevalent large percentage of P. aeruginosa isolates exhibited high levels of resistance to meropenem (20)% and the lowest level to Imipenem (5.5)%. Molecular analysis showed the phz M and phz S genes identified in all of the carbapenem resistance P.aeruginosa. Conclusions: Large percentage of P. aeruginosa isolates exhibited high levels of resistance to meropenem (20%) and the lowest percentage to Imipenem (5.5%). The results showed that all isolates have phenazine genes (Phz S, Phz M).