Microbial inoculants are an essential tool for increasing arable land productivity and reducing the application of mineral fertilizers. The purpose of this work was to isolate and identify endophytic antagonistic bacteria from the roots of medicinal plants and to assess their antifungal activity, and plant growth-promoting traits. Bacillus subtilis and Proteus mirabilis were isolated from roots of two native medicinal plants (Urginea martime and Atriplex lindlyi) from flora Arish and characterized by 16S rRNA gene sequencing. In vitro, El antagonistic activity experiments against (Fusarium oxysporum and Rhizoctonia solani) were evaluated. Our results revealed that the percentage of growth inhibition by B. subtilis and P. mirabilis were 96% and 78.5 % against R. solani followed by 85.3 % and 77% against F. oxysporum, respectively. we investigated by Screening electron microscope (SEM). Both endophytic bacteria B. subilits and P. mirabilis produced protease and chitinase. while also producing sidrophores and ammonia, but P. mirabilis capable to phosphate solubilization, and B. subtilis is fixing N₂. That promotes plant growth and facilitates bio control. These findings imply that this bacterial strain offers excellent protection against diseases of various agriculturally significant plants via direct and indirect modes of action.