Guava fruits are subjected to infect by several plant pathogens derange the storage as a post-harvest diseases. Anthracnose is one of the most prevalent fruit rot diseases. This study focused on pathogens of anthracnose, which infected guava fruits at post-harvest. Pathogenicity, host range, morphological and physiological characteristics were studied. Colletotrichum gloeosporiodes, and Pestalotiopsis psidii were isolated from guava fruits. Clementina cv was more susceptible to P. psidii and C. gloeosporiodes infection, while pepper, cucumber, and zucchini fruits were resistant to P. psidii. Navel orange and beans are less susceptible to infect by C. gloeosporiodes. The temperature from 25-30°C was the optimum degree for the growth of C. gloeosporiodes, while the temperature from 30-35°C was optimum for P. psidii. The appropriate pH for all fungi under study was 6.5, followed by 6 pH. PDA was the appropriate solid medium for the growth of C. gloeosporiodes, while Richard^'s Agar was the most favored for growth of P. psidii.