The experimental work was conducted at the Experimental Farm of Ismailia Agriculture Research Station, Ismailia Governorate Egypt,  to study the  effect of iron ( 0.0, 7.5, 15  and 30 ppm) from Fe3O4-NPs,  Zn (0.0, 0.25, 0.50 and 1.00 g/liter) from ZnO-NPs  and  Titanium Dioxide TiO2-NPs  (0.0, 0.25, 0.50 and 1.00 g/liter)  as well as  treated  of seeds pre-sowing  for 0, 5, 10 and 15-minutes to He-Ne Laser Irradiation on growth,  seed yield and active constituents of Silybum marianum plants during the two successive seasons of 2018 / 2019 and 2019 / 2020. All experiments were designed as randomized complete blocks design with three replicates. This work included four experiments: The most important results could be summarized as follows: Spraying Silybum marianum plants with Fe3O4-NPs at 30 ppm, ZnO-NPs at 1 g/l or TiO2-NPs at 1 g/l twice, firstly before flowering stage in 9th of February and secondly, after flowering stage in 9th of March in the two seasons. Also, irradiation of Silybum marianum seeds with He-Ne laser radiation (4 mW) for 15 minutes before sowing increased vegetative growth parameters, number of inflorescences/plant, dry weight of roots, herb and inflorescences/plant, seed yield/feddan., TXF, SDN, SBN, SCN, ISBN, total silymarin in fruits, chlorophyll a, chlorophyll b, total chlorophyll a+b, N, P and K contents in leaves, Fe, Zn and Ti concentrations in fruits.