The aim of the research is to study the effect of organic, bio fertilization and NPK fertilizers on the production of two varieties of cowpea under North Sinai conditions, to achieve this goal. This research was conducted in the Experimental Farm at the Faculty of Agricultural and Environmental Sciences-Al-Arish University, during two successive seasons (2020/2021) on two cultivars of cowpea (Karim 7 and Dokki-331), under treatments of fertilizers viz. organic (farmyard manure and chicken manure), bio fertilization (Effective microorganisms "ES1" and Technology of smart fertilizer "TS") and NPK fertilizers (Control 50%NPK, NPK 100%=100kg/fed, EM1+50%NPK, TS+50%NPK and their interactions). The experiment was laid out by using 16 treatments and three replications in Randomized Complete Block Design (RCBD). The data on vegetative characters viz. leaves number, plant height, peduncle number, nodules number, leaf area, root length, root number, leaves weight, root weight and shoot weight). The results cleared that the cultivar Karim-7 under EM1 bio- fertilizer + NPK 50% treatment was superior and significantly increased all studied traits.